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Table 2 Analysis of gene expression in PACs isolated from bone marrow of wild type (PPARΓ+/+) or PPARΓ haplodeficient mice (PPARΓ+/-) and cultured in vitro for 9 days

From: PPARγ activation but not PPARγ haplodeficiency affects proangiogenic potential of endothelial cells and bone marrow-derived progenitors

Gene

Normoxia

Hypoxia

Oxidative stress and cytoprotection

PPARΓ +/+

PPARΓ +/-

PPARΓ +/+

PPARΓ +/-

Nox4 (NOX4)

0,00065 ± 0,00012

0,00090 ± 0,00016

0,00052 ± 0,00008

0,00129 ± 0,00023*

Cyba (p22phox)

0,702 ± 0,102

0,748 ± 0,094

0,449 ± 0,077

0,829 ± 0,185

Mgst3 (GST III)

0,043 ± 0,005

0,060 ± 0,006*

0,043 ± 0,004

0,060 ± 0,005*

Inflammation

PPARΓ +/+

PPARΓ +/-

PPARΓ +/+

PPARΓ +/-

Tnfrsf1a (TNFR-1)

0,178 ± 0,028

0,235 ± 0,017* 0.092

0,132 ± 0,015

0,173 ± 0,012* 0.051##

Selplg (P-selectin ligand)

0,00018 ± 0,00008

0,00021 ± 0,00005

0,00019 ± 0,00004

0,00031 ± 0,00006

Extracellular matrix remodeling

PPARΓ +/+

PPARΓ +/-

PPARΓ +/+

PPARΓ +/-

Mmp2 (MMP-2)

0,070 ± 0,005

0,086 ± 0,010

0,044 ± 0,003##

0,101 ± 0,029* 0.081

Mmp9 (MMP-9)

0,416 ± 0,124

0,194 ± 0,067

0,263 ± 0,067

0,148 ± 0,033

Migration and proliferation

PPARΓ +/+

PPARΓ +/-

PPARΓ +/+

PPARΓ +/-

Nos3 (NOS3)

0,00049 ± 0,00006

0,00063 ± 0,00013

0,00044 ± 0,00005

0,00061 ± 0,00009

Cav1 (caveolin-1)

0,051 ± 0,006

0,080 ± 0,007**

0,104 ± 0,010##

0,162 ± 0,008**,###

Sdf-1 (CXCL12)

0,069 ± 0,021

0,110 ± 0,022

0,071 ± 0,022

0,088 ± 0,010

Cxcr4 (CXCR-4)

0,023 ± 0,007

0,023 ± 0,004

0,033 ± 0,006

0,037 ± 0,004#

Cxcr7 (CXCR-7)

0,0021 ± 0,0005

0,0021 ± 0,0003

0,0095 ± 0,0020#

0,0081 ± 0,0013##

Vegfa (VEGF-A)

0,052 ± 0,005

0,051 ± 0,005

0,185 ± 0,047#

0,164 ± 0,008###

Flt-1 (VEGFR-1)

0,00028 ± 0,00004

0,00038 ± 0,00011

0,00045 ± 0,00003#

0,00065 ± 0,00019

KDR (VEGFR-2)

0,0021 ± 0,0002

0,0027 ± 0,0007

0,0013 ± 0,0005

0,0019 ± 0,0003

  1. For last 24 h cell were incubated in normoxia (21% O2) or hypoxia (2% O2). Quantitative RT-PCR, EF2 serves as an internal control. Means ± SEM. N = 5-6, *p < 0.05, **p < 0.01 PPARΓ+/+versus PPARΓ+/-, #p < 0.05, ##p < 0.01, ###p < 0.001 normoxia versus hypoxia. For 0.05 < p < 0.1 exact p values are shown.