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Table 2 Analysis of gene expression in PACs isolated from bone marrow of wild type (PPARΓ+/+) or PPARΓ haplodeficient mice (PPARΓ+/-) and cultured in vitro for 9 days

From: PPARγ activation but not PPARγ haplodeficiency affects proangiogenic potential of endothelial cells and bone marrow-derived progenitors

Gene Normoxia Hypoxia
Oxidative stress and cytoprotection PPARΓ +/+ PPARΓ +/- PPARΓ +/+ PPARΓ +/-
Nox4 (NOX4) 0,00065 ± 0,00012 0,00090 ± 0,00016 0,00052 ± 0,00008 0,00129 ± 0,00023*
Cyba (p22phox) 0,702 ± 0,102 0,748 ± 0,094 0,449 ± 0,077 0,829 ± 0,185
Mgst3 (GST III) 0,043 ± 0,005 0,060 ± 0,006* 0,043 ± 0,004 0,060 ± 0,005*
Inflammation PPARΓ +/+ PPARΓ +/- PPARΓ +/+ PPARΓ +/-
Tnfrsf1a (TNFR-1) 0,178 ± 0,028 0,235 ± 0,017* 0.092 0,132 ± 0,015 0,173 ± 0,012* 0.051##
Selplg (P-selectin ligand) 0,00018 ± 0,00008 0,00021 ± 0,00005 0,00019 ± 0,00004 0,00031 ± 0,00006
Extracellular matrix remodeling PPARΓ +/+ PPARΓ +/- PPARΓ +/+ PPARΓ +/-
Mmp2 (MMP-2) 0,070 ± 0,005 0,086 ± 0,010 0,044 ± 0,003## 0,101 ± 0,029* 0.081
Mmp9 (MMP-9) 0,416 ± 0,124 0,194 ± 0,067 0,263 ± 0,067 0,148 ± 0,033
Migration and proliferation PPARΓ +/+ PPARΓ +/- PPARΓ +/+ PPARΓ +/-
Nos3 (NOS3) 0,00049 ± 0,00006 0,00063 ± 0,00013 0,00044 ± 0,00005 0,00061 ± 0,00009
Cav1 (caveolin-1) 0,051 ± 0,006 0,080 ± 0,007** 0,104 ± 0,010## 0,162 ± 0,008**,###
Sdf-1 (CXCL12) 0,069 ± 0,021 0,110 ± 0,022 0,071 ± 0,022 0,088 ± 0,010
Cxcr4 (CXCR-4) 0,023 ± 0,007 0,023 ± 0,004 0,033 ± 0,006 0,037 ± 0,004#
Cxcr7 (CXCR-7) 0,0021 ± 0,0005 0,0021 ± 0,0003 0,0095 ± 0,0020# 0,0081 ± 0,0013##
Vegfa (VEGF-A) 0,052 ± 0,005 0,051 ± 0,005 0,185 ± 0,047# 0,164 ± 0,008###
Flt-1 (VEGFR-1) 0,00028 ± 0,00004 0,00038 ± 0,00011 0,00045 ± 0,00003# 0,00065 ± 0,00019
KDR (VEGFR-2) 0,0021 ± 0,0002 0,0027 ± 0,0007 0,0013 ± 0,0005 0,0019 ± 0,0003
  1. For last 24 h cell were incubated in normoxia (21% O2) or hypoxia (2% O2). Quantitative RT-PCR, EF2 serves as an internal control. Means ± SEM. N = 5-6, *p < 0.05, **p < 0.01 PPARΓ+/+versus PPARΓ+/-, #p < 0.05, ##p < 0.01, ###p < 0.001 normoxia versus hypoxia. For 0.05 < p < 0.1 exact p values are shown.