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Table 1 Analysis of transcriptome of PACs isolated from bone marrow of wild type (WT) or diabetic (db/db) mice and cultured in vitro for 9 days

From: PPARγ activation but not PPARγ haplodeficiency affects proangiogenic potential of endothelial cells and bone marrow-derived progenitors

Gene Normoxia Hypoxia
Oxidative stress and cytoprotection WT db / db WT db / db
Nox4 (NOX4) 148 ± 20.3 245 ± 15.2* 153 ± 25.0 302 ± 123.8#0.082
Cyba (p22phox) 29331 ± 1383.1 35829 ± 1277.4** 28248 ± 2195.8 34380 ± 5610.4
Prkcd (PKCδ) 3134 ± 75.8 3698 ± 286.6* 3667 ± 518.5 3965 ± 413.3
Chst1 (GST-1) 6979 ± 411.2 4412 ± 341.3** 8339 ± 562.9 5774 ± 374.8*#
Mgst3 (GST III) 3388 ± 453.1 1741 ± 100.7* 4076 ± 590.9 2563 ± 342.6* 0.070; #0.091
Gpx4 (GPX4) 7819 ± 354 6920 ± 423.3* 8394 ± 76.9# 7599 ± 614.9* 0.076; #0.099
Inflammation WT db / db WT db/db
Tnfrsf1a (TNFR-1) 1259 ± 68.7 1447 ± 124.7* 0.051 1103 ± 298.5 1147 ± 82.5#
Csf3r (G-CSF3R) 188 ± 6.7 214.9 ± 9.5* 209 ± 7.9 237 ± 36.1
Cxcl16 (CXCL16) 2328 ± 215.3 3194 ± 403.5* 2464 ± 319.2 2972 ± 535.2
Selplg (P-selectin ligand) 1930 ± 219.1 2758 ± 324.4* 2737 ± 822.8 3214 ± 311.0# 0.077
Extracellular matrix remodeling WT db / db WT db / db
Mmp2 (MMP-2) 2825 ± 395.2 6498 ± 1373.3* 2567 ± 900.6 6454 ± 905.8**
Mmp9 (MMP-9) 1906 ± 1005.0 7554 ± 4171.7* 0.069 1713 ± 637.0 9625 ± 955.3***
Mmp14 (MMP-14) 5081 ± 513.8 5965 ± 461.9* 5499 ± 518.5 5758 ± 519.5
Mmp23 (MMP-23) 1625 ± 273.8 2127 ± 267.9* 2396 ± 521.2#0.054 2956 ± 338.2#
Migration and proliferation WT db / db WT db / db
Itgam (integrin-α1) 983 ± 254.4 1397 ± 30.8* 0.052 1499 ± 313.8#0.096 1397 ± 498.6*
Itgb2 (integrin-β2) 354 ± 37.4 438 ± 30.9* 472 ± 76.2# 587 ± 90.8* 0.085#
Itgb4 (integrin-β4) 105 ± 11.2 141 ± 4.28* 178 ± 66.0# 0.096 187 ± 26.7#
Ngef (NGEF) 163 ± 2.9 104 ± 8.2** 246 ± 36.2# 140 ± 5.7*##
Cct2 (CCT2) 166 ± 4.0 139 ± 4.9** 136 ± 11.6# 145 ± 16.6
Msx1 (MSX-1) 184 ± 18.5 132 ± 13.7** 159 ± 17.8#0.081 127 ± 1.24*
Cdkl2 (CDKL2) 233 ± 17.3 177 ± 7.4** 205 ± 19.7#0.066 193 ± 15.9
Areg (AREG) 322 ± 69.7 118 ± 14.5* 290 ± 126.6 155 ± 39.4* 0.099
Mdk (midkin) 236  ± 6.9 313 ± 21.6** 184 ± 3.1### 300 ± 17.9**
Nos3 (NOS3) 122 ± 3.0 142 ± 5.2** 99 ± 7.4# 106 ± 6.3###
Cav1 (caveolin-1) 4279 ± 675.2 2171 ± 242.9* 6460 ± 418.0## 4889 ± 968.4*#
Fgf7 (FGF-7) 7073 ± 780.0 5858 ± 795.5* 0.066 5736 ± 403.8# 5306 ± 958.3
Ang2 (angiogenin) 198 ± 50.5 138 ± 15.9* 0.085 164 ± 42.4 131 ± 6.1
Vegfb (VEGF-B) 1637 ± 107.1 1641 ± 91.4 2396 ± 97.9### 2195 ± 246.8#
Vegfc (VEGF-C) 714 ± 69.2 524 ± 91.5* 730 + 111.0 904 ± 331.3# 0.089
Vegfd (VEGF-D) 791 ± 50.7 499 ± 105.6* 1011 ± 289.9 839 ± 113.8##
  1. For last 24 h cell were incubated in normoxia (21% O2) or hypoxia (2% O2).
  2. Means ± SEM of normalized fluorescence signal. N = 3 for each gene, *p < 0.05, **p < 0.01 WT versus db/db, #p < 0.05, ##p < 0.01, ###p < 0.001 normoxia versus hypoxia. For 0.05 < p < 0.1 exact p values are shown.
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Cardiovascular Diabetology

ISSN: 1475-2840