Figure 9

Effect of induction of hind limb ischemia in wild type (WT) and PPARΓ haplodeficient (PPARΓ^+/-) mice. A: Number of lymphocytes (Ly), granulocytes (Gr) and monocytes (Mo) in the peripheral blood of WT and PPARΓ^+/- mice before (d 0) and 1 day (d 1) after surgery. Automated hematology analyzer. B: Leukocytic infiltration of gastrocnemius muscle before (d 0) and 14 and 28 days (d 14 and d 28) after surgery. Semiquantitative analysis and representative pictures. Hematoxylin-eosin staining. C: Percentage of regenerating fibers in ischemic muscle, 28 days after surgery. Numerical data and representative picture. Hematoxylin-eosin staining. D: Blood flow expressed as a ratio between ischemic and control leg. Numerical data and representative pictures. Laser-Doppler flowmetry. E: Number of capillaries in the ishcmic muscle, 28 days after surgery. Immunohistochemical staining for CD31. Mean + SEM. N = 5-9 (9A-C, E), N = 10 (9D), *p < 0.05, **p < 0.01, ***p < 0.001 versus d 0; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus WT.