Skip to main content
Figure 4 | Cardiovascular Diabetology

Figure 4

From: PPARγ activation but not PPARγ haplodeficiency affects proangiogenic potential of endothelial cells and bone marrow-derived progenitors

Figure 4

Effect of oral daily administration of PPARγ agonist rosiglitazone (ROSI, 10 mg/kg of body weight, 14 days) on endothelial progenitor cells and expression of proangiogenic genes in diabetic (db/db) mice. Control db/db and wild type (WT) mice were treated with vehicle. A: Percentage of CD45-KDR+Sca-1+ cells in bone marrow. B: Percentage of CD45-KDR+Sca-1+ cells in peripheral blood. C: Number of CD45-KDR+Sca-1+ cells in peripheral blood. Multicolor FACS phenotyping. D: Expression of VEGF mRNA in bone marrow. Quantitative RT-PCR. EF2 serves as an internal control. E: Concentration of VEGF protein in bone marrow. F: Concentration of VEGF protein in peripheral blood. ELISA. G: Expression of SDF-1 mRNA in bone marrow. Quantitative RT-PCR. EF2 serves as an internal control. H: Concentration of SDF-1 protein in bone marrow. I: Concentration of SDF-1 protein in peripheral blood. ELISA. J: Expression of PPARγ mRNA in bone marrow. Quantitative RT-PCR. EF2 serves as an internal control. Each bar represents mean + SEM. N = 8-10 (4A-C, F, I), N = 4-6 (4D, E, G, H, J), *p < 0.05, **p < 0.01, ***p < 0.001 versus WT, #p < 0.05 versus db/db.

Back to article page