Angiogenic potential of PACs and endothelial cells from wild type (WT) and diabetic (db/db) mice. A: Proliferation of PACs. Immunohistochemical detection of PCNA-positive cells. B: Migration of PACs in modified Boyden chambers. C: Formation of sprouts from PACs spheroids embedded in collagen gel, shown as a total length of sprouts per one spheroid. D: Formation of cords by PACs seeded on Matrigel shown as a number of cord junctions per microscopic field. E: Formation of capillaries by endothelial cells from aortic rings isolated from WT or db/db mice and plated on Matrigel. F: Formation of cords by HUVECs seeded on Matrigel and stimulated with empty medium (NC, negative control) or conditioned media from WT PACs and db/db PACs, shown as a number of cord junctions per microscopic field. Each bar represents mean + SEM. N = 4-5 (1A-E), N = 8-9 (1F), *p < 0.05, **p < 0.01 versus WT, ###p < 0.001 versus NC.