GW3965 reduced diabetes-induced cardiac fibrosis. a. Representative image of Sirius Red-stained LV sections, 20 ×μmagnification. b. Pooled data on quantification of collagen area per visual field (n =8-9). c-d. TGF-β1 and collagen 1A1 gene expression was determined by real-time PCR. Results were normalized against GAPDH and converted to fold induction relative to db/+ mice (n =6). *P < 0.05 or **P < 0.01 vs db/+ group; #P < 0.05 or ##P < 0.01 vs db/db group. Abbreviations: GW, GW3965; LV, left ventricle; TGF-α1, transforming growth factor beta1; collagen 1A1, collagen, type I, alpha 1.