Figure 6

Effect of cariporide on methylglyoxal-induced upregulation of endothelial adhesion molecule expression. (A) Representative original Western blot and means ± SEM (n = 4) showing the expression of P-selectin, E-selectin and ICAM-1 (relative to β-actin) determined in whole cremaster muscle 4 h after an intrascrotal injection of saline (Control), MG (50 mg/kg) alone or co-treated with MG and cariporide (20 mg/kg, 1 h prior to MG). * indicates significant difference (p < 0.05) from absence of MG (Control). # indicates significant difference (p < 0.05) from MG alone. (B) Representative confocal micrographs of cell surface ICAM-1 immunostaining (Red) in EE2 ECs in the absence (Control) or in the presence of MG (100 μM, 4 h) alone or co-treatment with MG and cariporide (50 μM, 1 h prior to MG). Nuclear staining is shown in blue (Hoechst 33342).