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Figure 3 | Cardiovascular Diabetology

Figure 3

From: Endothelial Na+/H+ exchanger NHE1 participates in redox-sensitive leukocyte recruitment triggered by methylglyoxal

Figure 3

Contribution of oxidative stress in methylglyoxal-triggered endothelial NHE1 activation. (A) Means  ± SEM (n = 6) of DCF-dependent fluorescence in EE2 ECs treated with MG (100 μM for 0-8 h) alone (MG) or co-treated with MG and cariporide (50 μM; 1 h prior to MG) or Tempol (300 μM; 1 h prior to MG). * indicates significant difference (p < 0.05) from MG alone. (B) Representative original Western blot and means  ± SEM (n = 4) showing total NHE1 and phospho-NHE1 (14-3-3 binding motif at p-Ser703; relative to β-actin) determined in EE2 ECs treated with MG (100 μM for 1 h) alone or co-treated with MG and cariporide (50 μM; 1 h prior to MG) or Tempol (300 μM; 1 h prior to MG). * indicates significant difference (p < 0.05) from the absence of MG (Control). # indicates significant difference (p < 0.05) from MG alone.

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