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Figure 2 | Cardiovascular Diabetology

Figure 2

From: Endothelial Na+/H+ exchanger NHE1 participates in redox-sensitive leukocyte recruitment triggered by methylglyoxal

Figure 2

Methylglyoxal-triggered endothelial SGK-dependent NHE1 activation. A - B. Representative original Western blot (A) and means  ± SEM (B; n = 4) showing total NHE1 (white bars) and phospho-NHE1 (14-3-3 binding motif at p-Ser703; black bars; relative to β-actin) determined in the absence (Control) or in the presence of MG-treatment (100 μM for 4 h) in EE2 ECs without (MG) or with the SGK1 inhibitor GSK650394 (20 μM, 1 h prior to MG). * indicates significant difference (p < 0.05) from the Control (0 μM MG). # indicates significant difference (p < 0.05) from MG alone. C - E. Representative original Western blot (C) and means  ± SEM (n = 4) showing total NHE1 (D) and phospho-NHE1 (14-3-3 binding motif at p-Ser703; relative to β-actin; E) determined in MG-treated (100 μM for 0-8 h) EE2 ECs with scrambled siRNA (Control siRNA; black bars) or with SGK-targeted siRNA silencing (white bars). * indicates significant difference (p < 0.05) from 0 h. # indicates significant difference (p < 0.05) from scrambled control siRNA.

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