Figure 5
From: Exendin-4 ameliorates cardiac ischemia/reperfusion injury via caveolae and caveolins-3
Caveolin-3 expression and reduction in infarct size. Mice underwent 30-min coronary artery occlusion followed by 2-h reperfusion after 24-h recovery from pretreatment with oxygen (Control) or exendin-4 (Ex-4) in wild-type and caveolin-3 knockout (Cav-3 KO) mice. (A) In vivo Ex-4 induced cardiac protection protocol. (B) Area at risk was calculated as a percentage of the left ventricle and revealed no significant differences between all groups. Ex-4 induced cardiac protection was abolished Cav-3 KO mice, as shown by no significant decrease in percent infarct size / area at risk when compared to control Cav-3 KO; however, a significant decrease in infarct size was observed between wild-type Ex-4 and Cav-3 KO Ex-4. (C) Cardiac troponin I, a marker of myocardial damage also revealed a significant decrease in Ex-4 treated control mice, but no effect in Cav-3 KO mice. Group sizes are indicated on the individual bars in parentheses. *P < 0.001 compared with Cav-3 KO pretreated with Ex-4.