Figure 4
From: Exendin-4 ameliorates cardiac ischemia/reperfusion injury via caveolae and caveolins-3
Lysed and fractionated hearts on sucrose density gradient. Fractions were collected and probed for caveolin-3 (Cav-3). We defined fractions 4-6 as buoyant membrane fractions enriched in caveolae and proteins associated with caveolae; fractions 9-12 were defined as nonbuoyant fractions, noncaveolar membranes. Fraction 7-8 were considered a transition zone and were not analyzed. Significant localization of Cav-3 in buoyant fractions was observed in the groups treated with exendin-4 (Ex-4), whereas control and methyl-μ-cyclodextrin (MβCD)-treated with Ex-4 cells showed no effects on Cav-3 localization. Group sizes are indicated on the individual bars in parentheses.