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Figure 3 | Cardiovascular Diabetology

Figure 3

From: Exendin-4 ameliorates cardiac ischemia/reperfusion injury via caveolae and caveolins-3

Figure 3

Glucagon-like peptide-1 receptor (GLP-1R) localization with caveolins or caveolae. (A) Immunofluorescence analysis of the expression and colocalization of caveolin-3 (Cav-3) and GLP-1R in cardiac myocytes. Fluorescent secondary antibodies were used to determine Cav-3 (green) and GLP-1R (red) localization, and strong colocalization (merged images, yellow) were observed on the cell surface membrane. Bar length = 10  μm. (B) The colocalization was confirmed by immunoprecipitation (IP). Immunoblot (IB) analysis detected Cav-3 and GLP-1R in Cav-3 and GLP-1R IP of cell lysates. Supernatants (SUP) from which the immunoprecipitates were generated were stained for GAPDH as loading controls.

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