Figure 6
From: p38 mitogen-activated protein kinase is involved in arginase-II-mediated eNOS-Uncoupling in Obesity
p38mapk is involved in Arg-II-induced eNOS-uncoupling. (A) In cultured HUVECs, overexpressing Arg-II gene led to elevated p38mapk activation as measured by the Thr180/Tyr182 phosphorylated p38mapk (p-p38mapk). n = 6, **p < 0.01 vs Con group. (B) HUVECs were transduced first with rAd/U6-LacZshRNA as control or rAd/U6-p38shRNA. Twelve hours after the 1st transduction with the rAd/U6-shRNAs, the cells were then transduced either with rAd/CMV as control (Con) or with rAd/CMV-Arg-II to overexpress Arg-II (Arg-II). Experiments were performed 60 hours post the 2nd transduction (48 hours in 5% FCS-RPMI-1640 medium plus overnight serum-starvation in 0.2% FCS-RPMI-1640). Shown are representative images of four independent experiments. ***p < 0.005 vs Con/LacZ group, †††p < 0.005 vs Arg-II/LacZ group. Scale bar = 0.2 mm.