Role of iNOS in the inhibition of UCP-2 expression level induced by TNF-α. (A) qRT-PCR analysis of iNOS mRNA expression in ECs and VSMCs stimulated with TNF-α for 2, 4 and 8 hours. (B) Western blot analysis of UCP-2 levels with or without iNOS inhibitor (L-NAME) in ECs and VSMCs stimulated with TNF-α. *p < 0.05 vs. control; †p < 0.05 vs. stimuli. qRT-PCR analysis of iNOS mRNA expression in aorta from Control and ApoE−/− mice at 8, 12, 18 and 24 wk of age (C) and Control and BATIRKO mice under high-fat diet (D). *p < 0.05 vs. Control; †p < 0.05 vs. BATIRKO MH mice.