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Figure 1 | Cardiovascular Diabetology

Figure 1

From: Antagonistic effect of TNF-alpha and insulin on uncoupling protein 2 (UCP-2) expression and vascular damage

Figure 1

Effect of insulin, oleate and TNF-α on UCP-2 expression level in vascular cells. (A) The effect of insulin on UCP-2 expression levels in ECs and VSMCs was analyzed by Western blot. (B) Effect of TNF-α on the phosphorylation of Akt, p44/42 and p70S6K in both vascular cell lines stimulated by insulin. (C) Effect of TNF-α pretreatment on UCP-2 protein levels in vascular cell lines stimulated by insulin. (D) qRT-PCR analysis of UCP-2 mRNA expression in vascular cell lines stimulated by oleate. (E) Effect of TNF-α pretreatment on UCP-2 expression in vascular cell lines stimulated by oleate. β-Actin was used as loading control. *p < 0.05 vs. control; † p < 0.05 vs. stimulus.

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