Figure 2

Induction of ICAM-1 expression in coronary endothelial cells by PGN and LPS requires TLR2 and TLR4, respectively. A. Coronary endothelial cells isolated from TLR2 KO and wild type (C57BL/6) mice were treated with PGN (10 μg/ml) for 24 h. A representative immunoblot shows that PGN induced a robust increase in ICAM-1 levels in wild type cells, but it had a minimal effect on ICAM-1 levels in TLR2 KO cells. B. A representative immunoblot shows that induction of ICAM-1 expression by LPS (200 ng/ml, 24 h) is markedly reduced in coronary endothelial cells from TLR4-defective (C3H/HeJ) mice.