Figure 1

Diabetic CAECs express higher levels of ICAM-1 after stimulation with PGN and LPS. A and B. Non-diabetic and diabetic CAECs were stimulated with PGN (10 μg/ml) or LPS (200 ng/ml) for 24 h, and ICAM-1 protein levels were analyzed by immunoblotting. Diabetic cells exhibited a greater increase in ICAM-1 protein levels. C. Non-diabetic and diabetic CAECs were stimulated with PGN (10 μg/ml) or LPS (200 ng/ml) for 2 h and analyzed for ICAM-1 mRNA levels by real time RT-PCR. Diabetic cells expressed higher levels of ICAM-1 mRNA. Results are expressed as Mean ± SEM; n = 5; *P < 0.05 vs. control; #P < 0.05 vs. non-diabetic cells treated with LPS or PGN.