R and RyR Protein Distribution Changes with High Glucose Treatment. Immunofluorescence experiments picture the relative location of each IP3R isoform in the cells grown in medium and high glucose. Few changes were noted in the distribution of IP3R-1 (A and B). There was a loss in fluorescence in IP3R-2 (C and D) and IP3R-3 (E and F) with high glucose treatment are shown. Cells grown in medium glucose conditions displayed primarily cytoplasmic staining of the RyR with perinuclear localization (G). RyR in cells grown in high glucose exhibited strong nuclear localization (H).