Intracellular Ca2+ Transients were Altered by Diabetes. Whole cell vasopressin and thapsigargin-induced Ca2+ transients were altered by diabetes in VSMC from the aorta and femoral artery. Vasopressin was applied to VSMCs from the aorta (A) or femoral artery (B) from DR-BB rats at time 0. The time of the thapsigargin application is shown with the arrow (TSG). In both cases the vasopressin-induced Ca2+ transients were blunted in amplitude and thapsigargin responses were delayed in cells from diabetic rats. For all figures, blue shades indicate data of cells from diabetic and control rats; green shades indicate data from cultured A7r5 cells grown in various glucose concentrations.