Figure 6

Sirt1 protein overexpression and p53 deacetylation in diabetic GK rat myocardium 12 weeks after MI. In (A) results from western blotting shows an increase in Sirt1-protein expression in the myocardium of GK rats 12 weeks after sham or MI operation. Results from immunohistochemistry show an increase in the number of Sirt1--positive nuclei from myocardial tissue sections in the infarct border zone (B). Panel (C) shows representative Sirt1-immunohistochemistry micrographs (×200) from posterior wall (sham and MI) and anterior wall (MI). Panel (D) shows a decrease in the acetylation of the Sirt1-target protein p53 in the myocardium of post-MI GK rats. The lower panels in (A) and (D) show representative western blots of myocardial tissue probed with anti-Sirt1, anti-tubulin, anti-p53 and anti-acetyl p53. Data is presented as means ± SEM; * indicates P < 0.05 vs. W (sham-operated Wistar), ^# indicates P < 0.05 vs. GK (sham-operated GK), ^† indicates P < 0.05 vs. W+MI.