Figure 6
From: Synergism in hyperhomocysteinemia and diabetes: role of PPAR gamma and tempol
Effect of acetylcholine (ACH) on contraction of cardiac muscle. Calcium chloride was applied on the cardiac ring (uniform transverse section of the LV) for maximum pre-contraction followed by dose dependant treatment of ACH. A. The upper and lower panels represent the contraction pattern of WT and Ins2+/-/CBS+/-, respectively. B. The percentage relaxation (indicating the potential of contraction) was calculated with respect to the maximum contraction after treatment with 20 mM CaCl2 and compared among WT, CBS+/-, Ins2+/- and Ins2+/-/CBS+/-. The capability of contraction decreased in the increasing order of CBS +/-, Ins2+/- and Ins2+/-/CBS+/- when compared to WT. Each data point represents mean ± SD of at least six animals.