Figure 4

Irbesartan inhibited the high glucose-induced EndMT in HAECs according to laser-scanning confocal microscopy. Representative immunofluorescence staining of CD31 (green) and FSP1 (red) were observed. A merging of both images reveals populations of cells acquired FSP1 expression and lost CD31 expression (arrows, B). The administration of irbesartan reduced the number of co-localization of CD31 and FSP1 (C, P < 0.05). A: normal glucose as controls; B: Treated with HG (30 mM) for 48 h. C: Treated with HG (30 mM) + irbesartan (1 μM) for 48 h. Experiments were repeated three times. NG: normal glucose. HG: high glucose. HI+Irb: high glucose + Irbesartan.