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Figure 1 | Cardiovascular Diabetology

Figure 1

From: Angiotensin II mediates the high-glucose-induced endothelial-to-mesenchymal transition in human aortic endothelial cells

Figure 1

The dose and time dependency of HG-stimulated Ang II synthesis in HAECs. A: HAECs grown in a culture medium consisting of 5.5 mM, 15 mM, or 30 mM glucose for 48 h. Mannitol was used as a control for hyperosmolarity. B: HAECs grown in a 30 mM glucose medium for 6-72 h. Ang II was measured in the supernatant using radioimmunoassay. C: High glucose stimulated Ang II synthesis in the supernatant in the absence or presence of irbesartan in HAECs. HAECs that were grown in a serum-free normal glucose medium (NG, 5.5 mM) for 24 h and exposed to high glucose (HG, 30 mM) or in 5.5 mM glucose + 24.5 mM mannitol (NG+M) in the absence or presence of 1 μM irbesartan for 48 h. Irbesartan partially inhibited Ang II production in the culture medium. The depicted values are the means mean ± SD from three independent experiments that were performed in duplicate. # P < 0.05 vs. mannitol (A) or NG (B). *P < 0.05 vs. HG without inhibitor.

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