Table 1 Primers used for the determination by real-time PCR of mRNA concentrations:
Name | Forward primer | Reverse primer | Size (bp) |
|---|---|---|---|
In rats | Â | Â | Â |
ACC1 | caacgcaggcatcagaa | caagtattccacagtccc | 138 |
ChREBP | cgggacatgtttgatgactatgtc | aataaaggtcggatgaggatgct | 86 |
FAS | ggtgctacccattcgtg | ggatgtatcattcttggactt | 115 |
Srebp-1c | cgctaccgttcctctatcaa | ttcgcagggtcaggttctcc | 164 |
VLDLr | tctggagttcctagctcat | ccagtgaatttattggcacc | 108 |
FAT | aggaagtggcaaagaat | tgaaggctcaaagatgg | 155 |
LPL | cctgaagacacagctgagga | cacccaactctcatacattc | 141 |
In mice: | Â | Â | Â |
ACC1 | cgctggtcttagaagttga | tccctgcygatgtatttgat | 149 |
ChREBP | gtccgatatctcgacacactc | cattgccaacataagcatgttctg | 91 |
FAS | tgctgccgtgtccttctacca | gcacccaagtcctcgccata | 128 |
Srebp-1c | ggcactaagtgccctcaacct | gccacatagatctctctgccagtgt | 81 |
FATP | cctgcggcttcaaca | tcagtggctccatcgt | 84 |
FAT | ggaactgtgggctcattgc | catgagaatgcctccaaacac | 68 |
In humans | Â | Â | Â |
ACC1 | acatccctacgctaaaca | agaacatcgctgacacta | 85 |
ChREBP | tcggcaatgctgacatg | gaggcgggagttggtaaa | 98 |
FAS | acggccctcatttccag | tgaagctcacccagttatcc | 87 |
Srebp-1c | tgaagacagacggagcca | ggactgttgccaagatggtt | 120 |
18S (mice, Rat, human) | tgaggccatgattaagaggg | agtcggcatcgtttatggtc | 190 |