Mutations in the coding regions of the hepatocyte nuclear factor 4 alpha in Iranian families with maturity onset diabetes of the young

Table 1 Nucleotide sequences of DNA primers used for PCR amplification of the HNF4α gene

Region	Sense primer (5'→3')	Antisense primer (5'→3')	Segment size	Tannealing/°C
1	tgtaaaacgacggccagtgggcactgggaggaggcagt	caggaaacagctatgacccttggcaacacctgtgctggc	405 bp	75
1b	tgtaaaacgacggccagttcatatcagcaacatgtccg	caggaaacagctatgaccgggctcttccctccagga	210 bp	73
2	tgtaaaacgacggccagtcttcctgaagcctcactcc	caggaaacagctatgacccccaagtgtgcccatttcc	352 bp	75
3	tgtaaaacgacggccagtgttgtgtcttctccatcca	caggaaacagctatgaccgcaggtggggcagtggtg	215 bp	56
4	tgtaaaacgacggccagttctccctcctcacctctctg	caggaaacagctatgacccctctgtagtgtggggga	226 bp	56
5	tgtaaaacgacggccagtatctccagcattttcttccc	caggaaacagctatgacccactgcccactactgccc	267 bp	72
6	tgtaaaacgacggccagtagggtacagatggcaaacac	caggaaacagctatgaccaccctccctggagccctg	204 bp	70
7	tgtaaaacgacggccagttgacttcccatcctccctcc	caggaaacagctatgaccggagagagagtcagggatgg	268 bp	68
8	tgtaaaacgacggccagtagctggaccctgctgccc	caggaaacagctatgacccactccaaccccgcccct	354 bp	74
9	tgtaaaacgacggccagtgcatcccagactctccatcc	caggaaacagctatgaccttgcaaggtaaaatcccagag	262 bp	72
10	tgtaaaacgacggccagtagcccctgtctgtctgtttg	caggaaacagctatgaccgggactggtcctggcatcac	316 bp	72
Val/Met255 variant	ccggagctggcggagatgacccg	caggaaacagctatgaccggagagagagtcagggatgg	180 bp	70

ISSN: 1475-2840