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Figure 7 | Cardiovascular Diabetology

Figure 7

From: Changes in cultured endothelial cell glycosaminoglycans under hyperglycemic conditions and the effect of insulin and heparin

Figure 7

Correlation of Total HS Disaccharides and Total GAGs in Cultured Endothelial Cells. Control cells and cells treated with high glucose (30 mM) were cultured for 24, 48 and 72 hours (3 dishes/group). Total GAGs were isolated, after cells and medium separately from the three dishes were determined by the carbazole assay. HS disaccharides were obtained by heparinase digestion and determined by RPIP-HPLC systems.

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