Figure 3From: Changes in cultured endothelial cell glycosaminoglycans under hyperglycemic conditions and the effect of insulin and heparinGAGs from Cultured Endothelial Cells and Medium at 72 Hours. Cells were treated with high glucose (30 mM) and/or insulin (0.01 unit/ml) for 72 hours. Total GAGs were isolated from cells (white bar) and medium (grey bar) and determined by the carbazole assay. Three dishes/group. Data were analyzed by a one-way ANOVA. Significantly different than: a, Control; b, Glucose; c, Insulin. p < 0.05.Back to article page