Table 1 Primers used for semiquantitative estimation of gene expression
gene | acronym | forward (5' > 3') | reverse (3' > 5') | bp |
|---|---|---|---|---|
cyclophilin A | Ppia | CTGAGCACTGGGGAGAAAGGA | GAAGTCACCACCCTGGCACA | 87 |
perilipin | Plin | GAGGGGCTGATCTGGCTTTG | GCATCTTTTGCCGTCCTGAA | 102 |
leptin | Lep | CGGTTCCTGTGGCTTTGGT | CCGACTGCGTGTGTGAAATG | 130 |
adiponectin | Adipoq | GGAGACGCAGGTGTTCTTGG | AGCCCTACGCTGAATGCTGA | 152 |
resistin | Retn | TCATGCCCAGAACCGAGTTG | CAGCCCCAGGACAAGGAAGA | 109 |
tumor necrosis factor | Tnf | GGCTCCCTCTCATCAGTTCCA | CGCTTGGTGGTTTGCTACGA | 104 |
visfatin | Pbef1 | TCTGGAAATCCGCTCGACAC | CACTCCGTCCCCTTGAATGA | 129 |
CD68 | Cd68 | CCATCCCCACTTGGCTCTCT | TGCGCTGAGAATGTCCACTG | 156 |
MCP1 | Cc12 | TGCAGGTCTCTGTCACGCTTC | TTCTCCAGCCGACTCATTGG | 148 |
interleukin 1β | Il1b | ATGCCTCGTGCTGTCTGACC | GGCCACAGGGATTTTGTCG | 130 |
interleukin 6 | Il6 | TGCCTTCTTGGGACTGATGTTG | TGGTCTGTTGTGGGTGGTATCC | 97 |
VEGF-A | Vegfa | CGTCCTGTGTGCCCCTAATG | TGTGCTGGCTTTGGTGAGGT | 124 |
PAI-1 | Serpine1 | CAGCACACAGCCAACCACAG | GAAGCGAACCCTTTCCCAAA | 143 |