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Table 4 Kinase-dependent MYPT1 phosphorylation (phospho-treonine) in aortic samples from normoglycemic (group N), normoglycemic in vivo losartan treated (group NL), diabetic (group D) and diabetic in vivo losartan treated (group DL) rats.

From: Losartan counteracts the hyper-reactivity to angiotensin II and ROCK1 over-activation in aortas isolated from streptozotocin-injected diabetic rats

MYPT1 phosphorylation (Arbitrary Units)

Groups

No drug addition

+HA-1077

+ Y-27632

N

2.02 ± 0.27

1.19 ± 0.10

(Δ: 0.83 ± 0.18)

0.72 ± 0.068

(Δ: 1.3 ± 0.136)

NL

1.99 ± 0.135

0.99 ± 0.05

(Δ: 1.0 ± 0.3)

0.56 ± 0.03

(Δ: 1.43 ± 0.29)

D

3.4 ± 0.18***,§§

0.47 ± 0.109

(Δ:2.92 ± 0.28***;§§

0.72 ± 0.04

(Δ: 2.67 ± 0.25*)

DL

2.17 ± 0.083

0.52 ± 0.171

(Δ: 1.65 ± 0. 24)

0.63 ± 0.039

(Δ: 1.55 ± 0.11)

  1. ***P < 0.001 vs. N and NL; §§P < 0.01 vs. DL; *P < 0.05 vs. N, NL and DL. Samples from rat aortas were prepared as described in "Methods". Values represent the mean ± SEM of 4 rat aortas from each group.
  2. Δ = difference between the MYPT1 phosphorylation measured in the absence of drug addition and that obtained in the presence of indicated ROCK1 inhibitors.
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Cardiovascular Diabetology

ISSN: 1475-2840