Gene polymorphism |
Forward primer (FP) Reverse primer (RP) | PCR cycling conditions | Restriction endonuclease | PCR product (bp) | Restriction fragment size (bp) |
---|---|---|---|---|---|
A20C |
FP-5'-AGA GGT CCC AGC GTG AGT GTC-3' RP-5'-AGC CCA CAG CTC AGT TAC ATC-3' | 95°C- 3 m | EcoO109I[10] | 265 | A-265 C-205,137 |
94°C- 1 m | |||||
64°C -1 m | |||||
72°C -1 m | |||||
72°C-5 m | |||||
X 30 | |||||
MboI |
FP-5'-GAG GTT CGA GTC GGC CCC CT-3' RP-5'-TGC CCA AAC ATG GCC ACA CAT-3' | 94°C- 5 m | MboI[30] | 250 | MboI (-) -250 |
94°C- 30 s | MboI (+) -171, 79 | ||||
68°C- 30 s | |||||
72°C- 30 s | |||||
72°C-5 m | |||||
X 30 | |||||
Lys173Arg |
FP- 5'-AGG CAG CTT CTA CCA GGG CCC CAG TCA CT-3' RP- 5'-CCC CTC CCC TGC AAA TCT CAT CCC TTA-3' | 94°C- 3 m | Bsu36I[31] | 1286 | L-1286, A- 1037, 249 |
94°C -45 s | |||||
61°C -45 s | |||||
72°C -1 m | |||||
72°C-10 m | |||||
X 35 |