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Table 1 Oligonucleotides for amplification and screening for three polymorphisms using PCR-RFLP method

From: Analysis of renin-angiotensin aldosterone system gene polymorphisms in malaysian essential hypertensive and type 2 diabetic subjects

Gene polymorphism Forward primer (FP)
Reverse primer (RP)
PCR cycling conditions Restriction endonuclease PCR product (bp) Restriction fragment size (bp)
A20C FP-5'-AGA GGT CCC AGC GTG AGT GTC-3'
RP-5'-AGC CCA CAG CTC AGT TAC ATC-3'
95°C- 3 m EcoO109I[10] 265 A-265 C-205,137
  94°C- 1 m    
  64°C -1 m    
  72°C -1 m    
  72°C-5 m    
  X 30    
MboI FP-5'-GAG GTT CGA GTC GGC CCC CT-3'
RP-5'-TGC CCA AAC ATG GCC ACA CAT-3'
94°C- 5 m MboI[30] 250 MboI (-) -250
  94°C- 30 s    MboI (+) -171, 79
  68°C- 30 s    
  72°C- 30 s    
  72°C-5 m    
  X 30    
Lys173Arg FP- 5'-AGG CAG CTT CTA CCA GGG CCC CAG TCA CT-3'
RP- 5'-CCC CTC CCC TGC AAA TCT CAT CCC TTA-3'
94°C- 3 m Bsu36I[31] 1286 L-1286, A- 1037, 249
  94°C -45 s    
  61°C -45 s    
  72°C -1 m    
  72°C-10 m    
  X 35    
  1. PCR cycling conditions are represented as temperature and time [minute (m), seconds (s] of initial denaturation, denaturation, annealing, extension and final extension X number of cycles.