Figure 1

Expression of α _v β ₃ integrins by HASMC and effect of α _v β ₃ antagonists on cell adhesion and apoptosis. Results of a representative FACS experiment (A) and data from 5 independent experiments (B) are shown (* = p < 0.05 vs no antibody or vs 10E5). HASMC growth-arrested for 72 hours were treated with insulin (1 μmol/L) or vehicle ± integrin inhibitors. 48 hours later (120 hours after the addition of quiescent media), Annexin V staining was determined (C). The groups are as follows: 1 – no inhibitor, 2 – 10E5, 3 – m7E3, 4 – c7E3, 5 – LM609, 6 – eptifibatide, 7 – tirofiban, 8 – cRGD, 9 – RGD, 10 – RGE and 11 – RAD. HASMC in suspension were incubated with eptifibatide, RGE peptides, m7E3 or 10E5 and then added to non-coated tissue culture plates. Cell adhesion was determined as described in Methods (D). Concentrations of inhibitors were as follows: m7E3, c7E3, LM609 and 10E5 (30 μg/ml); eptifibatide, GRGDS, GRDGS or GRADSP peptides (10 μmol/L) and tirofiban (30 μmol/L).