Figure 5From: Exercise training enhanced myocardial endothelial nitric oxide synthase (eNOS) function in diabetic Goto-Kakizaki (GK) ratsExercise training decreased LV eNOS-mRNA levels. Total LV RNA was isolated and quantification of mRNA levels was done by QRT-PCR as described in Methods. Data was normalized by the Δ2Ct method using β-actin, and the fidelity of the reactions was verified by melting point analysis. Values presented are % control mean ± SEM of 10 animals per group; * p < .05 compared control.Back to article page