Rosiglitazone prevents Nrf2-depedent CD-36 up-regulation in hypercholesterolemic C57BL/6J mice. (a) CD36 mRNA relative expression in pooled liver samples from analyzed groups normalized to GAPDH expression. *P < 0.05. (b) ChIP with an Nrf2 antibody of study groups was used to amplify a CD36 promoter fragment. ChIP figures percentages indicate increments or decrements regarding C57BL/6J mice fed HFHC diet. Data of C57BL/6J mice fed chow diet was established as 100%. (c) Quiascent day-5 adipocytes incubated with RG and LST were treated with ox-PACP for additional 24 h. RNA was isolated to measure adipose protein 2 by conventional PCR and CD36 expression using real-time PCR. Values were normalized to GAPDH expression. Figure bar plots represent mean ± SEM percentage from vehicle-treated cells of at least 4 experiments with duplicate measurements.