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Table 1 Primers and thermal profiles for real-time RT-PCR.

From: Maternal diabetes induces congenital heart defects in mice by altering the expression of genes involved in cardiovascular development

Gene

GenBank Accession No.

Primer

Product size (bp)

Annealing temp./time (°C/s)

Extension temp./time (°C/s)

β-actin

NM_007393

5'-tgttaccaactgggacgaca-3'

5'-ggggtgttgaaggtctcaaa-3'

165

55/7

72/20

Bmp4

NM_007554

5'-tgatacctgagaccgggaag-3'

5'-agccggtaaagatccctcat-3'

190

55/8

72/20

Msx1

NM_010835

5'-agctctgctgccctatacca-3'

5'-cttggcctctgcatccttag-3'

205

57/8

72/20

Pax3

NM_008781

5'-ctgcactcaagggactcctc-3'

5'-gtgaaggcgagacgaaaaag-3'

169

57/7

72/20