Gene | GenBank Accession No. | Primer | Product size (bp) | Annealing temp./time (°C/s) | Extension temp./time (°C/s) |
---|---|---|---|---|---|
β-actin | NM_007393 | 5'-tgttaccaactgggacgaca-3' 5'-ggggtgttgaaggtctcaaa-3' | 165 | 55/7 | 72/20 |
Bmp4 | NM_007554 | 5'-tgatacctgagaccgggaag-3' 5'-agccggtaaagatccctcat-3' | 190 | 55/8 | 72/20 |
Msx1 | NM_010835 | 5'-agctctgctgccctatacca-3' 5'-cttggcctctgcatccttag-3' | 205 | 57/8 | 72/20 |
Pax3 | NM_008781 | 5'-ctgcactcaagggactcctc-3' 5'-gtgaaggcgagacgaaaaag-3' | 169 | 57/7 | 72/20 |