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Table 1 Primers and thermal profiles for real-time RT-PCR.

From: Maternal diabetes induces congenital heart defects in mice by altering the expression of genes involved in cardiovascular development

Gene GenBank Accession No. Primer Product size (bp) Annealing temp./time (°C/s) Extension temp./time (°C/s)
β-actin NM_007393 5'-tgttaccaactgggacgaca-3'
5'-ggggtgttgaaggtctcaaa-3'
165 55/7 72/20
Bmp4 NM_007554 5'-tgatacctgagaccgggaag-3'
5'-agccggtaaagatccctcat-3'
190 55/8 72/20
Msx1 NM_010835 5'-agctctgctgccctatacca-3'
5'-cttggcctctgcatccttag-3'
205 57/8 72/20
Pax3 NM_008781 5'-ctgcactcaagggactcctc-3'
5'-gtgaaggcgagacgaaaaag-3'
169 57/7 72/20