Effect of TZDs on the induction of thymidine kinase assessed by acute [3H]-thymidine uptake and parallel estimation of [3H]-thymidine incorporation into DNA. A. Confluent serum deprived vSMCs were treated with PDGF with and without troglitazone for 20 h then cells were washed free and growth factor and drugs and the acute uptake of [3H]-thymidine over 4 min was assessed. The results show the effects of two identical experiments in duplicate. B. Shows concomitant data for routine assay of [3H]-thymidine into DNA (see methods for details).