Table 1 List of sequences of PCR primers used

a.

Gene

Restriction Enzyme

Direction

Primers

CAPN10 (A/G-iSNP43)

Nsp I

Sense

5' GCTGGCTGGTGACATCAGTG3'

Antisense

5' TCAGGTTCCATCTTTCTGCCAG3'

ADRB3 (Try64Arg)

Mva I

Sense

5' CGCCCAATACC-GCCAACAC3'

Antisense

5' CCACCAGGAGTCCCATCACC3'

PPARγ (Pro12Ala)

Bst UI

Sense

5' GCCAATTCAAGCCCAGTC3'

Antisense

5'GATATGTTTGCAGACAGTGTATCAGTGAAGGAATCGCTTTCCG3'

FATP4 (Gly209Ser)

Hpa II

Sense

5' GTGAGGTCCATGCCAGCCTG3'

Antisense

5' CACCTGTGAAGCCCTTGGTCAG3'

b.

Gene

Restriction Enzyme

Direction

Primers

GPx-1 (Pro197Leu)

Hae III

Sense

5' TTATGACCGACCCCAAGCTCA3'

Antisense

5' ACAGCAGCACTGCAACTGCC3'

Catalase (SNP-89)

Hinf I

Sense

5'AATCAGAAGGCAGTCCTCCC3'

Antisense

5'TCGGGGAGCACAGAGTGTAC3'

Mn-SOD (Ala16Val)

BsaW I

Sense

5' GCTGTGCTTTCTCGTCTTCAG3'

Antisense

5'TGGTACTTCTCCTCGGTGACG 3'

Cu/Zn-SOD (intron 3)

Hha I

Sense

5'CTATCCAGAAAACACGGTGGGCC3'

Antisense

5'ATTGCCCAAGTCTCCAACATGC 3'

NADPH oxidase (+242)

Rsa I

Sense

5'ACACTGAGGTAAGTGGGGGTGGCTCCTGT3'

Antisense

5' TGCTTGTGGGTAAACCAAGGCCGGTG3'