Table 2 mRNA expression for ADAM and MMP genes in response to increasing human LDL concentrations (25,50 and 100 μg/ml from control or Type 2 diabetes donors) after 4,8 and 24 hours culture

MMP – 1

4 hrs

0.48 (0.09)

0.97 (0.21)

0.41 (0.14)

0.66 (0.11) *

0.29 (0.06)

0.62 (0.09) **

8 hrs

0.69 (0.08)

0.63 (0.07)

0.59 (0.05)

0.53 (0.08)

0.53 (0.07)

0.56 (0.05)

24 hrs

-

-

-

-

-

-

MMP – 9

4 hrs

0.98 (0.09)

1.36 (0.10) *

1.02 (0.15)

1.55 (0.13) *

1.01 (0.19)

1.98 (0.2) **

8 hrs

0.84 (0.05)

0.89 (0.07)

0.76 (0.05)

0.94 (0.05) *

0.73 (0.10)

0.94 (0.07) **

24 hrs

0.89 (0.06)

1.09 (0.06)

0.63 (0.04)

1.0 (0.07) ***

0.67 (0.09)

1.03 (0.08) **

TIMP – 1

4 hrs

0.73 (0.04)

0.88 (0.05)

0.74 (0.04)

0.86 (0.04)

0.73 (0.07)

0.90 (0.05) *

8 hrs

0.94 (0.06)

0.87 (0.04)

0.90 (0.05)

0.86 (0.03)

0.81 (0.04)

0.81 (0.04)

24 hrs

1.09 (0.06)

1.14 (0.07)

0.94 (0.06)

1.08 (0.05)

1.1 (0.10)

1.16 (0.06)

TIMP – 3

4 hrs

0.87 (0.04)

0.84 (0.04)

0.85 (0.03)

0.73 (0.04) **

0.79 (0.02)

0.70 (0.04)

8 hrs

0.95 (0.04)

0.90 (0.06)

0.85 (0.04)

0.84 (0.04)

0.75 (0.04)

0.68 (0.040

24 hrs

0.99 (0.06)

0.87 (0.04)

1.01 (0.07)

0.84 (0.04) **

0.96 (0.05)

0.86 (0.03)

ADAM – 28

4 hrs

0.76 (0.05)

1.07 (0.05)

0.83 (0.11)

1.07 (0.09)

0.78 (0.1)

1.23 (0.06) **

8 hrs

0.84 (0.06)

0.81 (0.04)

0.87 (0.07)

0.83 (0.05)

0.72 (0.05)

0.75 (0.06)

24 hrs

0.97 (0.07)

1.10 (0.07)

0.85 (0.05)

1.15 (0.09) *

0.95 0.11

1.06 (0.06)

ADAM – 17

4 hrs

0.93 (0.10)

1.19 (0.08)

0.81 (0.09)

1.13 (0.08) *

0.84 (0.12)

1.11 (0.06) *

8 hrs

0.94 (0.04)

1.03 (0.05)

0.93 (0.06)

0.86 (0.04)

0.79 (0.05)

0.78 (0.04)

24 hrs

1.08 (0.09)

1.15 (0.07)

0.92 (0.06)

1.13 (0.07)

1.14 (0.13)

1.17 (0.60)

ADAM – 15

4 hrs

0.91 (0.05)

1.07 (0.10)

0.96 (0.09)

1.18 (0.08)

0.91 (0.09)

1.21 (0.06) **

8 hrs

0.99 (0.4)

1.01 (0.06)

1.08 (0.07)

0.95 (0.04)

0.86 (0.04)

0.87 (0.05)

24 hrs

0.87 (0.03)

0.92 (0.07)

0.86 (0.05)

0.87 (0.05)

0.94 (0.08)

0.99 (0.06)