Unsaturated FFA block apoptosis induced by saturated FFA. HMC were incubated for 24 or 48 h with 0.2 mM palmitate (Palm) or stearate (Ste) alone or in co-incubations with 0.2 mM of the unsaturated FFA oleate (Ole) or linoleate (Lin). Control cells were incubated with albumin alone. After 24 and 48 h, the level of (A) the cleaved fragment of caspase-3 and (B) cytoplasmic nucleosomal fragments were determined by ELISA and expressed relative to the 24 h control value. Data are mean ± SEM for n = 3 independent experiments in triplicate. **, P < 0.01 by ANOVA versus control.