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Figure 4 | Cardiovascular Diabetology

Figure 4

From: Saturated free fatty acids and apoptosis in microvascular mesangial cells: palmitate activates pro-apoptotic signaling involving caspase 9 and mitochondrial release of endonuclease G

Figure 4

Palmitate but not oleate increases caspase 9 enzyme activity. HMC were treated with palmitate (0.2 and 0.4 mM) or oleate (0.4 mM) for 24 and 48 h. Caspase 9 activity was then measured in HMC extracts adjusted for total protein using a luminometric assay with an LEHD peptide substrate as described in Materials and Methods. Data are mean ± SEM for n = 3 experiments in duplicate. **, P < 0.01, *, P < 0.05 versus control or oleate alone by ANOVA.

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