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Table 1 Primers used in PCR reactions.

From: Decreased expression of β1- and β2-adrenoceptors in human diabetic atrial appendage

Primer

Primer sequence 5'-3'

PCR product size (bp)

Annealing Temperature (°C)

MgCl2 (mM)

β1-ARs (sense)

236CGAGCCGCTGTCTCAGCAGTGGACA260

201

54

1,2

β1-ARs(antisense)

436GGTGGCCCCGAACGGCACCACCAGCA412

   

β2-ARs (sense)

2135ACTGCTATGCCAATGAGACC2154

463

59

1,2

β2-ARs(antisense)

2597TGGAAGGCAATCCTGAAATC2578

   

β-actin (sense)

1079AAGTACTCCGTGTGGATCGG1098

286

54–59

1,2–1,3

β-actin(antisense)

1364CACCTTCACCGTTCCAGTTT1345

   

β-actin (sense)

854CTCTTCCAGCCTTCCTTCCT873

513

54–59

1,2–1,3

β-actin(antisense)

1366GTCACCTTCACCGTTCCAGT1347

   
  1. Primers were designed based on published sequences in the National Center for Biotechnology Information GenBank database http://www3.ncbi.nlm.nih.gov/entrez/: β1-ARs accession number NM_000684) [24]; β2-ARs (accession number XM_004030); β-actin (accession number NM_001101). Subscript numbers refer to positions of bases within the published cDNA sequences.
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Cardiovascular Diabetology

ISSN: 1475-2840