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Figure 3 | Cardiovascular Diabetology

Figure 3

From: Dopamine D4 receptors inhibit proliferation and migration of vascular smooth muscle cells induced by insulin via down-regulation of insulin receptor expression

Figure 3

Effect of D 4 receptor on insulin-mediated A10 cell migration. A and B. Effect of the D4 receptor agonist PD168077 on insulin-mediated A10 cell migration. A10 cell migration was determined by transwell (A) and scratch-wound migration assay (B) after incubation with insulin (10-7 mol/L), alone, or in the presence of varying concentrations of PD168077 (PD, 10-10 mol/ - 10-6 mol/L). Results are expressed as migratory cell number or migration distance (n = 9, *P < 0.05 vs. control; #P < 0.05 vs. insulin alone). C and D. Specificity of D4 receptor action on insulin-mediated migration of A10 cells. A10 cells were incubated with vehicle (control), insulin 10-7 mol/L alone or in the presence of the D4 receptor agonist, (PD, PD168077, 10-7 mol/L) (insulin + PD) or both D4 receptor agonist (PD) and D4 receptor antagonist (L, L745870, 10-7 mol/L) (insulin + PD + L). A10 cell migration was determined by transwell (C) and scratch-wound migration assay (D). Results are expressed as migratory cell number or migration distance (n = 9, *P < 0.05 vs. control; #P < 0.05 vs. insulin alone).

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