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Figure 1 | Cardiovascular Diabetology

Figure 1

From: Dopamine D4 receptors inhibit proliferation and migration of vascular smooth muscle cells induced by insulin via down-regulation of insulin receptor expression

Figure 1

Expression of D 4 receptors in rat thoracic aorta and A10 cells. A: D4 receptor protein expression was determined by confocal microscopy in thoracic aorta from SD rats. The arteries were washed, fixed, and immunostained for D4 receptor and α-smooth muscle (SM)-actin as described in the Methods. Colocalization appears as yellow after merging the images of fluorescein isothiocyanate-tagged D4 receptor (red) and Alexa 568-tagged α-SM-actin (green). B: D4 receptor protein expression was determined in artery by immunoblotting. The 47 kDa band indicated the expression of D4 receptor protein (lane1 and lane2) in A10 cells and rat thoracic aorta because it was no longer visible when the antibodies were pre-adsorbed by the immunizing peptide (1/10 w/w, lane3 and lane4). C. D4 receptor mRNA expression was determined by RT-PCR. The 367 bp band indicated the expression of D4 receptor mRNA in A10 cells and in rat thoracic aorta (lane3 and lane4) because the band was not observed in the absence of template (lane1) or primers (lane2).

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