Figure 4

Effect of calpain inhibition on apoptosis in high glucose-stimulated HUVECs. HUVECs were incubated with normal glucose (NG, 5 mmol/L) or high glucose (HG, 30 mmol/L) in combination with calpain inhibitor-III (CI-III, 10 μmol/L) or infection with Ad-CAST for 48 h. (A, B) Caspase-3 activity. (C, E) Representative images for annexin V staining in HUVECs (Green). (D, F) Quantification of annexin V staining positive cells. Data are given as mean ± SD from 3 different experiments. ^*P < 0.05 vs. NG treated with vehicle or infected with Ad-gal. ^#P < 0.05 vs. HG treated with vehicle or infected with Ad-gal.