Effect of GKA on TGF-β mediated 35S-SO
incorporation into proteoglycans by endothelial cells. Cells treated with TGF-β (2ng/ml) +/-GKA (RO28-1675) 1-10μM for 24 h and SB431542 (3μM) was used as TβR1 antagonist. Media containing secreted proteoglycans was spotted on chromatography paper and CPC precipitated to assess radiolabel incorporation into proteoglycans. Panel A shows the quantitation by the CPC precipitation method and Panel B shows the resulting SDS-PAGE analysis. Results are mean ±SEM of data normalised to control from three experiments in triplicate, ##p<0.01 versus control and **p<0.01 versus TGF-β using 1-way ANOVA.