Effect of D-glucose on the phosphorylation of Smad2C in endothelial cells. A. Western blot of whole cell lysates from BAECs treated with glucose (5–25 mM) for 60 min. TGF-β (2 ng/ml) for 5 min was used as positive control. Cell lysate were were resolved on SDS-PAGE and transferred onto a PVDF membrane. The membrane was probed with anti-phospho-Smad2(Ser465/467). Also probed with anti-Smad2 and anti-GAPDH. Chemiluminescence was used to detect the proteins of interest. B. Histogram is a densitometric quantitation of 3 Western blots. Results **p < 0.01 treatments versus basal (5 mM) n = 3 experiments determined by one-way ANOVA.