The relationship between the rate of glucose consumption and D-glucose concentration. Endothelial cells treated with 2.5, 5, 15, 25 mM D-glucose −/+GKA and TFG-β. Basal represent normal levels of the rate of glucose consumption mM/well/hour in endothelial cells. In the presence of GKA (10 μM) there was no effect on the rate of glucose consumption. Results are the mean ± SEM from 3 experiments in triplicate **p < 0.01 versus basal, ***p < 0.001 versus basal determined using two-way ANOVA.