Effect of GKA on glucose concentration (A) and consumption (B) on rat MIN6 pancreatic beta cells. A. MIN6 cells treated with either 5 mM or 25 mM glucose medium conditions in the presence and absence of two glucokinase activators (RO28-1675 and Compound A) at 10 μM for 0–24 h. Direct D-glucose levels were measured in the medium for each treatment. Data presented from an experiment utilizing 3 wells/treatment. ##p < 0.01 = basal (24 h) versus basal (0 h) and ##p < 0.01 = treatment versus basal (24 h) determined using one-way-ANOVA on a per well basis. B. RO28-1675 and Compound A increased (p < 0.01) the rate of consumption of glucose by MIN6 cells treated with 5 mM and 25 mM glucose compared to basal levels of glucose consumption after 24 h. Results are **p < 0.01treatment versus basal, determined using one-way-ANOVA.