Validation of miRNA expression results by real time PCR . HUVEC were incubated for 24 h with HSA (200 μg/ml), AGE-HSA (200 μg/ml) and glucose (250 mg/dl). In addition, 10-10 mol/l calcitriol was given to the cells 1 h after stimulation for an additional 23 h. The miRNA set that included (A) miR-659, (B) miR-510, (C) miR-181C, (D) miR-411, (E) miR-126, (F) miR-15a, and (G) miR-20b was validated using real time PCR. Insets show miRNA microarray expression results. Data are expressed as mean ± SD of 4–5 independent experiments. *P < 0.05 compared to control group-HSA. **P < 0.05 compared with AGE-glucose.