Figure 6From: Osteocalcin attenuates high fat diet-induced impairment of endothelium-dependent relaxation through Akt/eNOS-dependent pathwayEffect of OCN on the phosphorylation of Akt and eNOS in cultured HUVECs. (A) HUVECs incubated with 100 ng/mL OCN or vehicle for the indicated times. (B) The effects of OCN on Akt phosphorylation. (C) The effects of OCN on eNOS phosphorylation. (D) HUVECs incubated with the indicated concentrations of OCN or vehicle for 1 h. (E) The effects of OCN on Akt phosphorylation. (F) The effects of OCN on eNOS phosphorylation. Mean ± SEM of four independent experiments are plotted. *P < 0.05, **P < 0.01 vs. control group. OCN, osteocalcin.Back to article page