Figure 3

Role of gasotransmitters and prostaglandins in the vasodilatory effect of exenatide. Inhibition of eNOS with 300 μM N_ω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) (A). Inhibition of CO production by blocking heme oxygenase enzyme with 10 μM Tin-protoporphyrin IX dichloride (B). Blocking H₂S production by inhibiting cystathionine-γ-lyase with 10 mM DL-Propargylglycine (PPG) (C). Inhibition of prostaglandin production with 3 μM indomethacin (D). 23.9, 71.7, 310, 788, 1980, 3170 nanomoles of exenatide were used to relax the vessels (n = 5 of each experiment), *P < 0.01 compared to the relaxation caused by exenatide only (at respective concentration of exenatide).